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Determination of cellular glutathione (GSH)
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Cellular GSH was carried out according to the procedure as previously described with some modifications38. Briefly, HepG2 cells were treated with 2.5 mM AA in the presence or absence of BBE or BBD for 24 h, followed by incubating with 50 μg/mL NDA at 37 °C for 30 min. Cells were washed with PBS after incubation with the fluorescence probe and then evaluated immediately by fluorescence microscope. The results were expressed as mean NDA fluorescence intensity.
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