Immunohistchemical staining of macrophages in sensor implanted tissue using anti-mouse F4/80 antibodies

Ulrike Klueh; Izabela Ludzinska; Caroline Czajkowski; Yi Qiao; Donald L. Kreutzer

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Immunohistchemical staining of macrophages in sensor implanted tissue using anti-mouse F4/80 antibodies

UK Ulrike Klueh

IL Izabela Ludzinska

CC Caroline Czajkowski

YQ Yi Qiao

DK Donald L. Kreutzer

This method is extracted from research article: J Biomed Mater Res A, Sep 2017

Cross-linked basement membrane based coatings enhance glucose sensor function and continuous glucose monitoring in vivo

DOI: 10.1002/jbm.a.36206

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To confirm the observations of the presence of macrophages in tissue sections, we utilized a mouse macrophage specific antibody designated anti-mouse F4/80. Anti-mouse F4/80 (@F4/80) (Invitrogen Catalog # A14800) was validated using mouse spleen tissue and standard immunohistochemical (IHC) techniques (9).

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