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Cells were seeded into 96-well plates, and different concentration of cDDP was added into each well. Approximately 48 h after treatment, 20 μL MTS (promega) was added into each well, and the plate was incubated at 37°C for 4 h. The absorbance at 490 nm of each well was recorded by microplate reader BioTek Synergy 2. Growth rate was calculated as the ratio of the absorbance of the experimental well to that of the control.
Copyright and License information: ©2016 Jia et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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