Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
(iii) Mutation of ScAad3p.
Request a Protocol
Ask a
question
Favorite
PCR-based site-direct mutagenesis was performed to mutate cystine73 (coded by TG218C) into a presumed functional tyrosine73 (coded by TAC) in a manner similar to that described above for PcAad1Cys76p, except that primer set ScAad3MutCys2Tyr_B1/ScAad3MutCys2Tyr_C1 was used against template vector pGS21a-ScAad3. The resulting constructs were screened by colony PCR using the primer set AAD3_pGS_F/AAD3_pGS_R, validated by Sanger sequencing, and then named pGS21a-ScAad3Cys73Tyr.
Copyright and License information: ©2017 Yang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
