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Cyclooxygenase enzyme inhibition assay
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COX inhibition effect was tested by commercial colorimetric COX inhibitor screening assay kit (Cayman Chem., Co., Cat# 760111). In brief, 150 μl of assay buffer, 10 μl of heme, 10 μl of COX-2 enzyme and 20 μl of G-4 at concentration of 1, 3, 10, 30, and 100 μM were added into a 96-well plate. The plate was shaken carefully and the absorbance of each well was measured at 590 nm using a plate reader (VICTOR X3, PE, MA).
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