Cell proliferation assay and colony formation assay

For cell proliferation assay, cells were seeded in 96‐well plates at 2.0 × 10³cells/well in a final volume of 100 μL and incubated overnight. The viability of cells was determined with CellTiter 96 non‐radioactive cell proliferation assay (MTS) (Promega BioSciences, Madison, Wisconsin, USA) following the manufacturer's protocol. For colony formation assay, cells were placed in a six‐well plate and maintained in RPMI‐1640 supplemented with 10% FBS for 2 weeks. The colonies were fixed with 4% paraformaldehyde, stained with 0.1% crystal violet and counted.