Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

SEM observation

JH Jingchao Hu
YC Yu Cao
YX Yilin Xie
HW Hua Wang
ZF Zhipeng Fan
JW Jinsong Wang
CZ Chunmei Zhang
JW Jinsong Wang
CW Chu-tse Wu
SW Songlin Wang
request Request a Protocol
ask Ask a question
Favorite

Ex vivo expanded hDPSC sheets grown for 7–10 days were fixed using 2.5 % glutaraldehyde in 0.1 mol/l sodium cacodylate buffer (pH 7.2) for 2 h at 4 °C. After washing with sodium dimethylarsenate buffer, the cells were post-fixed in 1 % osmium tetroxide, dehydrated with gradient alcohol, and then incubated with isoamyl acetate. After gold coating, five samples were examined using a Hitachi S-520 scanning electron microscope (Hitachi, Tokyo, Japan).

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A