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Cell Sense (CS-1000 ATM, Celsense Inc.) was infused via a vented vial spike (ICU Medical) into the bioreactor. Reagent was either pumped in via the bioreactor pump or fed by gravity. Vials are filled with 27 mL of reagent, with the spike 25 mL being delivered per vial. Cells were labeled at 10 mg/mL CS-1000 ATM for the final 24 hours of culture.
Copyright and License information: ©2017 Charles F. O'Hanlon et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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