siRNA-mediated knockdown

RS Ramasri Sathanoori
PB Paulina Bryl-Gorecka
CM Christa E. Müller
LE Laurie Erb
GW Gary A. Weisman
BO Björn Olde
DE David Erlinge
request Request a Protocol
ask Ask a question
Favorite

Transient transfections using scrambled and P2Y 2-specific siRNA were performed using Lipofectamine RNAiMax as per manufacturer’s instructions and as previously published [12]. At 3 days post-transfection, the cells were either subjected to shear stress or exposed to the receptor agonists (ATPγS, UTP, and MRS-2768) and harvested for protein or processed for immunocytochemistry.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A