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siRNA-mediated knockdown

RS Ramasri Sathanoori
PB Paulina Bryl-Gorecka
CM Christa E. Müller
LE Laurie Erb
GW Gary A. Weisman
BO Björn Olde
DE David Erlinge
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Transient transfections using scrambled and P2Y 2-specific siRNA were performed using Lipofectamine RNAiMax as per manufacturer’s instructions and as previously published [12]. At 3 days post-transfection, the cells were either subjected to shear stress or exposed to the receptor agonists (ATPγS, UTP, and MRS-2768) and harvested for protein or processed for immunocytochemistry.

Copyright and License information: The Author(s) ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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