Transient transfections using scrambled and P2Y 2-specific siRNA were performed using Lipofectamine RNAiMax as per manufacturer’s instructions and as previously published [12]. At 3 days post-transfection, the cells were either subjected to shear stress or exposed to the receptor agonists (ATPγS, UTP, and MRS-2768) and harvested for protein or processed for immunocytochemistry.
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