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4.5. Measurement of Serum and Tissue Iron

XL Xin Li
PW Peina Wang
QW Qiong Wu
LX Lide Xie
YC Yanmei Cui
HL Haiyan Li
PY Peng Yu
YC Yan-Zhong Chang
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The total iron concentration of serum and tissues (heart, liver, spleen, kidney, testis) was measured using Serum Iron Assay Kit and Tissue Iron Assay Kit from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China) according to the manufacturer’s instructions. The serum was obtained by retro orbital bleeding, and the supernatant was collected after centrifugation at 2000× g for 20 min at 4 °C.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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