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The algae lipid extracts were analyzed by GC-MS on total fatty acids (TFAs) after transesterification of the lipids which contain fatty acids into the corresponding fatty acid methyl esters (FAMEs). The transesterification and GC-MS method were the same as previous research.37

The TFA yield is defined as

In this research, TFA fraction in crude lipid is also used for evaluation and it is defined as

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