The algae lipid extracts were analyzed by GC-MS on total fatty acids (TFAs) after transesterification of the lipids which contain fatty acids into the corresponding fatty acid methyl esters (FAMEs). The transesterification and GC-MS method were the same as previous research.37
The TFA yield is defined as
In this research, TFA fraction in crude lipid is also used for evaluation and it is defined as
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.