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Patient samples were incubated for 24 hours as described in “Cell viability assay.” Following incubation, the Promega Caspase-Glo 3/7 assay (Madison, WI) was used to quantitate caspase activity, as per the manufacturer’s protocol. Plates were read using a PerkinElmer Victor X Light Luminescence Counter.
Copyright and License information: ©2017 by The American Society of Hematology
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