Cell viability assay

Following isolation of the desired cell population, cells were incubated at a density of 100 cells per microliter in 100 μL of media, for a total of 10⁵ cells per well, in a black optical 96-well plate. Cells were incubated for 72 hours with venetoclax (3 nM to 100 µM), romidepsin (0.1-10 nM), and/or vorinostat (0.1-10 µM). The CellTiter-Glo Luminescent Cell Viability Assay (Promega, WI) was used to measure the number of viable cells in culture based on quantitation of the adenosine triphosphate (ATP) present. Plates were read using the Perkin Elmer Victor Light Luminescence Counter (Waltham, MA). Drug concentrations were applied in approximate half-log10 increments to patient samples, and in twofold increments for cell lines. Cell luminescence was normalized to a vehicle control containing 0.2% dimethyl sulfoxide (DMSO) and corrected for media.