Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Determination of intracellular ROS
Ask a
question
Favorite
Intracellular ROS were measured using a ROS assay kit. After the above designated treatment, the cells were harvested and incubated with 10 μmol/L of DCFH-DA (a fluorescent probe, which may be oxidized by ROS in viable cells to 2’,7’-dichlorofluorescein, DCF) for 30 min at 37 °C. After washing three times with PBS, DCF fluorescence was quantified with a multi-detection microplate reader (485 nm excitation and 535 nm emission).
Copyright and License information: ©2017 The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
