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Flow cytometry analysis determined cell cycle
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HeLa and CaSki cells were harvested from 6-well plates after transfection. Harvested cells were washed, fixed, permeabilized, and suspended in 0.3 mL PBS containing PI (50 μg/mL) at 4°C for 30 min. The percentage of cells in the different stages was measured by Flow Cytometry (No. Moflo XDP, Beckman Coulter, California, USA) and was evaluated using FlowJO software.
Copyright and License information: ©2017 Pan et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
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