Actin cytoskeleton staining

To analyse the actin cytoskeleton, SK‐BR‐3 breast cancer cells were infected with Ad‐GPF or Ad‐GFP‐YB1 CTD for 48 h, fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X‐100 at room temperature for 10 min. Thereafter, cells were incubated with TRITC‐phalloidin in the dark. Staining with 4′,6‐diamidino‐2‐phenylindole (DAPI) was used to visualize nuclear localization. Fluorescence microscopy was performed using the BX53 Microscope Systems (Olympus, Japan).