Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Peptide cleavage assays and HPLC analysis

TB Tillmann Bedau
NS Neele Schumacher
FP Florian Peters
JP Johannes Prox
PA Philipp Arnold
TK Tomas Koudelka
OH Ole Helm
FS Frederike Schmidt
BR Björn Rabe
MJ Marlene Jentzsch
PR Philip Rosenstiel
SS Susanne Sebens
AT Andreas Tholey
SR Stefan Rose-John
CB Christoph Becker-Pauly
request Request a Protocol
ask Ask a question
Favorite

Peptide cleavage assays were performed as described before [9]. In short, synthetic peptides were obtained from Genosphere Biotechnologies and analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC). The peptide solution (500 μM) was either analyzed alone or after preceding incubation with recombinant meprin β (15 nM) at room temperature. The digestion was stopped after each timepoint by addition of 0.1% trifluoroacetic acid. Collection of eluting fractions corresponding to chromatogram peaks was performed for subsequent mass spectrometry-assisted peptide analysis.

Copyright and License information:  ©2017 Bedau et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A