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Glutathione reductase activity was estimated by the method of Mateen S et al., 2016 [23]. To 0.2 ml of sample, 1.5 ml of buffer, 0.5 ml EDTA, 0.2 ml oxidized glutathione (GSSG) and 0.1 ml NADPH were added. The decrease in optical density of the enzyme was measured at 340 nm. The enzyme activity was calculated in terms of moles of NADPH oxidized/min/mg protein.
Copyright and License information: ©2017 Journal of Clinical and Diagnostic Research
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