Sequencing of 16S rRNA genes and bacteria identification

Bacterial isolates were identified by 16S rRNA gene sequencing. The gene from each bacterium was PCR amplified using the universal primers 27F and 1492R (Weisburg et al. 1991) and the first half (∼700–900 bp) of the product sequenced using primer 27F for identification at the genus level (Lo Giudice et al. 2007). The sequences have been deposited in GenBank under the accession numbers {"type":"entrez-nucleotide-range","attrs":{"text":"KT356815 to KT356839","start_term":"KT356815","end_term":"KT356839","start_term_id":"916445098","end_term_id":"916445122"}}KT356815 to KT356839 and {"type":"entrez-nucleotide-range","attrs":{"text":"KT356841 to KT356850","start_term":"KT356841","end_term":"KT356850","start_term_id":"916445124","end_term_id":"916445133"}}KT356841 to KT356850. Putative taxonomic identities were assigned using the RDP Bayesian classifier (Wang et al. 2007) with minimum bootstrap confidence of 80%.