2.8 Scratch assay

Scratch assay was performed according to standard procedures as described in ³². Each cell line was plated in 6-well plates (approximately 3 million cells/well) and incubated in growth medium for approximately 6 hours at 37°C to create a confluent monolayer. A straight line was scraped through the cell monolayer with a 200 µl pipet tip. Cells were then washed with PBS and cultivated in serum-free RPMI-1640. Microscope images (Motic, Wetzlar, Germany) were acquired directly after scratching and after additional 24 h incubation at 37°C.