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Primary cultures of neonatal rat ventricular cardiac myocytes (NRVMs) were prepared from 1–2 days old Wistar rats as described previously [18].
NRVMs were stimulated either with 10−7 M Angiotensin II (ANG II, Merck-Millipore, Darmstadt, Germany) or 10−4 M Phenylephrine (PE, Sigma Aldrich, St. Louis, MO, USA) to induce cellular hypertrophy.
Copyright and License information: ©2017 Schlegel et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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