Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
SW480 cells were resuspended in L15 + 10% FBS and seeded on 96-well plates coated with 5μg/ml fibronectin (FN). The plates were kept at 37°C under 5% CO2 for 45 minutes. Then cells were fixed with 50% glutaraldehyde for 30 minutes. Photographs were taken at 200× magnification using a phase contrast microscope (Nikon, Japan). Number of spread cells was counted.
Copyright and License information: ©2017 Wang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
