Unilateral Clamp Ischemia Model

Animal experiments were approved by the Indiana University School of Medicine Institutional Animal Care and Use Committee. Male Sprague Dawley rats (Harlan Laboratories) were anesthetized with isoflurane and placed on a warming pad for temperature maintenance. The right kidney was removed and the renal artery and vein sutured off. Ischemia of the left kidney was initiated by clamping the renal artery and vein for 30 minutes using nontraumatic clamps (Fine Scientific Tools). At the conclusion of the ischemic period, the clamps were removed and the kidneys were observed to insure rapid re-establishment of blood flow. The αvβ5 blocking antibody (ALULA) used in these studies was previously described.²³ The 2H6 mouse IgG2b mAb was used as a control. Antibodies were administered at a volume of 300 μl by subcutaneous injection at defined times before clamping.