Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
All samples were subjected to freeze-drying prior to derivatisation. 80 μl of methoxyamine hydrochloride in pyridine (20 mg/ml) was added to each sample and incubated at 30°C with shaking for 90 minutes. The samples were added with 160 μl of MSTFA and allowed to incubate at 37°C with shaking for 30 minutes. The samples were transferred to autosampler vials and allowed to acclimatise to room temperature for 2 hours prior to the GC-MS run.
Copyright and License information: ©2016 Liew et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
