Microarray Analysis and GSEA

Affymetrix Mouse Gene 2.0 ST arrays were used to assay RNA samples. Data was submitted to NIH GEO Accession number: {"type":"entrez-geo","attrs":{"text":"GSE95809","term_id":"95809"}}GSE95809. The 4-hour DHA treated samples were statistically tested by unequal variance t test to the DMSO control (3 replicates) and the volcano plot was generated by converting the resultant pvalue to a score (−log10(pvalue)) using Spotfire (TIBCO, CA). Gene set enrichment analysis (GSEA) (version 2.2.3) was performed using 4-hour DHA samples vs. DMSO control samples and the c5.all.v5.2 symbol.gmt Gene Ontology gene sets. Gene set permutation was performed 1000 times using the weighted enrichment statistic and genes ranked by signal to noise metric.