Endothelial cell in vitro assays

EA Egle Avizienyte
CC Claire L. Cole
GR Graham Rushton
GM Gavin J. Miller
AB Antonella Bugatti
MP Marco Presta
JG John M. Gardiner
GJ Gordon C. Jayson
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HUVEC proliferation, migration, sprouting and tube formation assays were performed as previously described [18, 21]. FGF2 and VEGF165 (Lonza) were used at 5–20 ng/ml and 2.5–20 ng/ml concentration, respectively. The treatment with synthetic dodecasaccharides in vitro was performed at 1, 10 and 50 μg/ml concentrations.

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