Antibody-dependent cellular cytotoxicity (ADCC)

The CytoTox 96 Nonradioactive Cytotoxicity assay (Promega, Madison, USA) was used for the cytotoxicity assay. Huh-7/BEL-7402 cells were co-cultured with various numbers of PBMCs or NK-92 cells in the presence or absence of treatment for 4 h at 37°C. LDH analysis was performed according to the manufacturer's protocol [12]. The controls were set as groups of spontaneous LDH release in effector and target cells and target maximum release. The calculation of cytotoxicity percentage was determined as: %Cytotoxicity = [(experimental - effector spontaneous - target spontaneous)/(target maximum - target spontaneous)]×100.