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Collagen (0.5 μg) was separated by 6% SDS-PAGE in the presence of 0.5 M urea. Gels were fixed in 45% methanol and 10% acetic acid, stained with 0.08 M picric acid, 0.04% Coomassie Brilliant Blue R250 (Sigma Aldrich) and destained in water. Versadoc3000 (BIO-RAD) and QuantityOne software (BIO-RAD) were used for gels digitalization and band densitometry.
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