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The leaves second from the top of the 4-week-old cotton plants were picked and cut into 4mm×5mm strips. For chlorophyll extraction, 0.10 g leaves were put into 50-ml tubes with a 25 ml ethanol–acetone mixture (volume ratio 1:1) in the dark for no more than 12 h until the leaves turned white. The extract was measured at absorbance values of 470, 645 and 663 nm by UV spectrophotometer, and the concentrations of chlorophyll a and chlorophyll b were measured using Arnon’s method (Arnon, 1949).
Copyright and License information: ©2017 The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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