Mice

The generation of Tr4 mutant mice through targeted deletion of the DNA-binding domain in embryonic stem cells followed by breeding into a mixed 129/SvJ genetic background was previously reported.³ Tr4^+/− mice were then backcrossed to C57BL/6 (CD45.2) mice for more than 7 generations. Genotyping was performed by PCR of tail biopsies or yolk sacs (oligos in Table 1). All animal experiments were reviewed and approved by the University Committee on Use and Care of Animals at the University of Michigan.

Sequences of qPCR and genotyping oligos

The gene name and sequences for the forward and reverse oligo sequences are listed for each primer pair used in these experiments for qPCR and genotyping.

qPCR, quantitative polymerase chain reaction.