2.1. Reagents and antibodies

DETA/NO, PAM₃CSK₄, LPS (Escherichia coli 0111:B4), 2-Deoxy-D-glucose (2-DG), oligomycin, rotenone and the mTOR inhibitor Rapamycin were from Sigma-Aldrich (St. Louis, MO). 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) was purchased through Life Technologies (Grand Island, NY). Western blotting antibodies anti-AMPKα (#2603) and anti-phospho-AMPKα (phospho Thr172) (#2535) were from Cell Signaling Technology (Beverley, MA). Anti-phospho-mTOR (phospho S2481) (#ab137133) antibody was from Abcam (Cambridge, MA). Anti-Actin (MAB1501) antibody was from Chemicon International (Temecula, CA). Human and mouse Enzyme-Linked Immunosorbent Assay (ELISA, IL-10 and TNFα) kits were purchased through eBioscience (San Diego, CA) and experimentation was performed per manufacturer's protocol.