Continuous variables were compared by one-way ANOVA with post hoc Tukey’s testing. Mortality was compared using log-rank analysis of Kaplan-Meier survival curves. Statistical significance was determined using a two-sided P value of 0.05. p50 values were calculated using nonlinear regression analysis. Statistical analysis was done with SPSS version 23 (IBM, Armonk, North Castle, NY).
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