Biofilm inhibition assay

Bacterial inocula were prepared and diluted 1:100 in fresh TSB+ broth as described in “Biofilm assay” Section. Inocula were supplemented with 10 µg mL⁻¹ proteinase K (Sigma-Aldrich) or 10 µg mL⁻¹ recombinant human DNase I (Genentech). Aliquots of inocula were transferred to microtiter plate wells, incubated for 24 h, and biofilm formation was quantitated by crystal violet staining.