Preparation of cellblock for histological analysis

The EUS-FNA specimen was placed in Cytolyte and taken to the laboratory where it was spun in the centrifuge. After decanting the supernatant, the sediment was made into a pellet and placed in a Tissue-Loc HistoScreen cassette (Microm International, Walldorf, Germany) and fixed in formalin. Thereafter, it was embedded in paraffin and sections made for hematoxylin and eosin (H&E) staining to examine for the presence of a histological core. Only the diagnostic specimens with definable histological core were included.