Bacterial strains and plasmids.

The strains, plasmids, and recombinant cells used in this study are listed in Table 2. All primers are listed in Table 3. C. pinatubonensis JMP134 was cultivated in lysogeny broth (LB) at 30°C. E. coli BL21(DE3) was used to produce the recombinant proteins in LB at 37°C. Kanamycin (50 mg/liter) was added as required. pET30 Ec/Lic (Novagen) was used as an overexpression vector for protein production and purification, and the low-copy-number plasmid pBBR1MCS2 (45) was used as an expression vector for physiological analysis.

Bacterial strains and plasmids used in this study

Oligonucleotide primers used for plasmid construction