2.2. Chemicals

Triciribine (EMD Chemicals, San Diego, CA) was dissolved in 100% DMSO (Sigma-Aldrich, St. Louis, MO) to a working stock concentration of 20 mM, aliquoted, and stored at −20 °C. Further dilutions to a final concentration of 0.15% DMSO were made to be controls for experiments using 30 µM. Mass Spectrometry experiments were performed using LC-MS grade acetonitrile (ACN) and water (J.T. Baker, Philipsburg, NJ). Formic acid from Pierce (Rockford, IL) and iodoacetamide (IAA) and dithiotheritol (DTT) were all purchased from Sigma-Aldrich (St. Louis, MO). Sequencing grade endoproteinase Glu-C was purchased via Promega (Madison, WI). GSK–3 recombinant enzyme was purchased from New England Biolabs (Product P6040S, Ipswitch, MA).