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Total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions, with minor modifications (3 M sodium acetate was used for the removal of polysaccharides). The quantity and purity of the total RNA were analysed using a NanoDrop 2000 spectrophotometer (Thermo scientific, USA) and an Agilent 2100 bioanalyser (USA) with the RNA Integrity Number (RIN) > 8.0. The total RNA was divided, stored at −80 °C, and used for high-throughput sequencing, qRT-PCR and RACE amplification.
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