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Cultured cells were collected together with their supernatant, and fixed with 80% ice‐cold ethanol. Following overnight incubation in −20°C, cells were washed twice with cold PBS, incubated for 10 min in 0.1% Triton solution (in PBS), followed by staining with DAPI (1 μg/ml). Samples were filtered to remove aggregates prior to analysis. DAPI area and width were measured by flow cytometry (BD Biosciences FACSCalibur). Images were analyzed using FlowJo software (Treestar, Ashland, OR).
Copyright and License information: ©2017 The Authors. Published under the terms of the CC BY 4.0 license
This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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