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Immunohistochemistry analysis

LS Lei Sun
JK Jia Ke
ZH Zhen He
ZC Zexian Chen
QH Qinghua Huang
WA Wenjia Ai
GW Guoqiang Wang
YW Yisheng Wei
XZ Xiangcai Zou
SZ Shi Zhang
PL Ping Lan
CH Chuyuan Hong
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Immunohistochemistry analysis was carried out according to the Envision System (Dako Cytomation, Glostrup, Denmark) guidance. In brief, each TMA slides was deparaffinzed and rehydrated through graded ethanol. Sodium citrate was used for antigen retrieval. Slides underwent 0.3% hydrogen peroxide solution to block endogenous peroxidase activity. Then samples was incubated with the primary antibody anti-HES1 (1:400; Abcam, ab71559), at 4°C overnight. After incubation with secondary (goat) antibody, slides were developed in diaminobenzine (EnVision, DAKO) and counterstained with haematoxylin.

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