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DNA from 200 uL of middle ear effusions was purified using the QiaAmp mini kit (Qiagen, Valencia, CA) prior to electrophoresis in 1% Agarose Gels run at 100 V in 1X TAE buffer containing ethidium bromide for visualization under ultraviolet lighting. Quantification of relative DNA concentrations in each effusion sample was performed by the Qubit double stranded DNA kit (Invitrogen, Carlsbad, CA).
Copyright and License information: ©2016 Val et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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