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The amounts of HMGB1 in the cell culture supernatants were detected using a double-antibody sandwich ELISA kit purchased from Wuhan Huamei Biotech Co. Ltd. (Wuhan, China). Operations were carried out according to the protocol provided by the manufacturer.
Copyright and License information: ©2017 Zhilin Qi et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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