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All DAB stained and emulsion coated slides were digitized with a Nikon Eclipse 80i microscope connected to a Nikon DXM-1200F digital camera (Nikon Corporation, Tokyo, Japan). Immunofluorescence images were taken by the Olympus FLUOVIEW FV1200 confocal microscope (Olympus Corporation, Tokyo, Japan). A maximum of 21 images of confocal z stacks were sectioned at 8 μm. Z projection of stacks series was averaged using ImageJ (1.46r). Adobe Photoshop CS4 (Adobe Systems, Mountain View, CA) was used to optimize the images and compose all figures.
Copyright and License information: ©2016 Okubo et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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