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Wound-healing and Transwell migration assay

ZS Zetao Shen
RZ Rui Zhou
CL Chen Liu
YW Yaofeng Wang
WZ Wanqi Zhan
ZS Ziyun Shao
JL Jian Liu
FZ Feifei Zhang
LX Lijun Xu
XZ Xinying Zhou
LQ Lu Qi
FB Feng Bo
YD Yanqing Ding
LZ Liang Zhao
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HCT116 and SW480 CRC Cells were seeded onto 24-well plates and grown to confluence. After starvation in serum-free medium for 12 h, cells were gently wounded using pipette tips. Images were taken at 0, 12, and 24 h after wounding. The motility of CRC cells was assessed through the distance between the wound edges. CRC cells cultured in 200 μl 1640 were seeded in the upper chamber of the Transwell insert, while 600 μl medium with 20% FBS was put in the lower chamber. Cells were cultured for 24 h and that invaded to the underside of the membrane were fixed and stained with crystal violet. Cells were counted from five random fields of view and the mean value was calculated from three independent experiments.

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