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Caspase-3/7 activity was measured with the Caspase-Glo 3/7 Assay Kit (Promega, Madison, WI, USA). Mesangial cells were seeded in a white-walled 96-well plate and then treated with palmitate. After 24 h, the cells were processed according to the manufacture’s instruction. GloMax Microplate Reader (Promega) was used for luminescence analysis.
Copyright and License information: ©2017 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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