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The hydrodynamic size, size distribution, and zeta potential are the three basic parameters for liposome systems. The size and zeta potential of LP were determined by dynamic light scattering (DLS) and electrophoretic light scattering (ELS), respectively, using ZetasizerNano ZS90 (Malvern Instruments Ltd., Malvern, UK) at 25 °C [29]. The measurement cycle was automatically determined by the instrument system. The particle size was presented by intensity distribution, and the size distribution was evaluated by polydispersity index (PDI).
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