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Preparation of alcohol-insoluble residue and cell wall monosaccharide analysis
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Alcohol-insoluble residue (AIR) was prepared from stem parts and hydrolyzed in 2 N trifluoroacetic acid for 1 h at 120 °C (Rautengarten et al., 2016). Monosaccharides were separated and quantified by high-performance anion exchange chromatography coupled with pulsed amperometric detection on an ICS 3000 instrument (Thermo Fisher Scientific) using a CarboPac PA20 anion exchange column (Thermo Fisher Scientific) as described previously (Rautengarten et al., 2016).
Copyright and License information: ©2017 The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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