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3.6. Agarose Gel Retardation Assay (Binging Capacity of Polymers/DNA Complexes)
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Agarose gel electrophoresis was performed to investigate DNA binding ability of P123-PEI-R4 and P123-PEI-R11. Polymer/DNA complexes at different weight ratios ranging from 0.05 to 3.0 were prepared. These obtained complex solutions were analyzed by electrophoresis with the condition of 120 V and 30 min on a 1% (w/v) agarose gel in loading buffer. Then these gels were stained with 0.5 g/mL ethidium bromide for 15 min and illuminated by a UV illuminator to show the location of DNA.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
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