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The apoptotic cells were measured by flow cytometry using an annexin V–FITC/propidium iodide apoptosis detection kit (Abcam, Cambridge, UK) in U2OS cells. The fluorescence intensity was detected at 488 nm using flow cytometry. Cells were sorted by the FACSCalibur flow cytometer (Becton Dickinson, San Diego,CA), and analyzed using CellQuest software (Becton Dickinson).
Copyright and License information: ©2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
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