Determination of antimicrobial peptide MICs.

Overnight cultures of bacteria in SPB (20 mM) supplemented with 0.1% TSB were diluted to 5 × 10⁵ CFU/ml in the same medium. Ninety microliters was transferred into 96-well microtiter plates containing 10 μl of serially diluted cyO2 solution. The plates were incubated in a shaking incubator at 37°C for 16 to 20 h. The tested concentration of cyO2 was 50 μM to 0.39 μM, and 1.5-fold dilution steps were used. At least two independent experiments, each with two technical replicates, were performed.