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NK cells were cultured in the presence of herbimycin A (250ng/mL), PDTC (500ng/mL), or neuraminidase (250ng/mL) at 28°C for 1h, followed by twice washings with PBS. Cells were then stimulated for 1 or 16h with NDV 7793 (25HU/105cells), recombinant HN (500ng/mL), or IFN-γ (500ng/mL). The presence of these drugs had no effect on lactate dehydrogenase (LDH) levels, a stable cytosolic enzyme released upon cell lysis, in NK cells.
Copyright and License information: ©2017 Liang et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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